The sensorial experiences enjoyed in the Ottoman and Safavid sacred spaces have not been given enough attention and yet stand as a curious research topic. While the subject has been investigated within the Ottoman context by Emine Fetvacı and Nina Ergin, who have introduced the multi-sensory aesthetic of Ottoman mosques and conceptualized them as venues offering different sensorial experiences, sensory aspects of Safavid religious spaces did not become the focus of any such research yet. This study intends to contribute to the limited literature on the sensory history of the early modern Islamic sacred spaces with a comparative case study of two early-seventeenth-century royal mosques. Focusing on Sultan Ahmed Mosque in Istanbul and Shah Mosque in Isfahan, it aims to reveal the sensory aspects of Ottoman and Safavid mosques and the sensorial-aesthetic appeals of their patrons and audiences. This inquiry argues that both mosques had designs pleasing different senses of their audiences, and offered multi-sensory experiences to their visitors. They had particular design elements, including eye-pleasing decorations, spatial harmony, and acoustic qualities, and were adorned with equipment such as incense burners and luxury lamps to appeal to the senses of sight, smell, touch, and hearing. Vocal practices and chanting sessions, including the recital of the Qur’an and the canonical texts in vernacular languages (Mevlid and Rawḍa), offered auditory experiences to worshippers frequenting these sanctuaries. Stiputalitons in their endowment deeds and the presence of luxury incense burners attest to the olfactory and haptic experiences offered to the visitors of these mosques. As a rather extraordinary practice within the context of Friday mosques, banquets offered to the participants of commemorative ceremonies, Mevlid and ‘Ashura, addressed the sense of flavor. This research relies on narrative sources, including endowment deeds, poems, and travelogues, as well as studies and observations on the buildings’ design and technical aspects. In this sense, it offers a multi-disciplinary approach combining the methods for the studies of history and architecture. Its multi-faceted framework and findings would similarly contribute to different disciplines investigating the functional-performative, aesthetic, and spatial-architectural, along with the sensorial aspects of the sacred spaces created in the early modern Ottoman and Safavid worlds.