The Coptic Orthodox Patriarch, Dīmitrīūs II, acted to end American missionary work in the south of Egypt in 1867. Starting in 1865, the American missionaries attempted to open new stations in the south, but the Coptic bishops opposed this move. Although they did not achieve a significant impact due to this resistance, the patriarch took a pastoral trip on a viceroy yacht and visited various dioceses, including three locations experiencing Protestant-Coptic conflict: Asyūṭ, Akhmīm, and Qūṣ. As he traveled, he opened Coptic schools, banned involvement with Protestant services and books, and excommunicated any Coptic-Protestants. He sometimes used physical force and the backing of governmental bodies against the indigenous converts to return them to the Coptic Church. In this context, the American missionaries concluded that the Coptic patriarch was acting against the policy of religious toleration promulgated by Ottoman Egypt, and they felt that the Egyptian government should stop him. In their writings, they named the conflict as “ecclesiastical warfare” and “Coptic opposition and persecution.” At the same time, the Coptic patriarch asserted a similar accusation against the American and Egyptian Protestants. The Ottoman Egyptian government supported the patriarch’s authority over his religious community as a plan to defend Coptic religious liberty against missionary attacks. Thus, this paper will ask the following question: How did their contrasting cultural views of religious toleration propel the American missionaries and Coptic leaders into ecclesiastical conflict? This historical study will analyze the cultural clash between the American missionaries and Coptic leaders in 1867. This research will benefit from recent scholarly developments in the historical study of Anglo-American Missions in Middle East in the 19th century. Several scholars, such as Heather Sharkey, Paul Sedra, and Ussma Makdisi have enriched the scholarship of American-European missions and Egyptian Christianity. They have highlighted the need for the re-reading of the history of Christianity and its context in distinctive ways. Moreover, they have investigated indigenous sources, marginalized groups and cities, powers, and conversion processes. My analysis will build on these studies, and present a new viewpoint about the Cultural encounters between the American missionaries, the Coptic Orthodox Church in the context of Ottoman Egypt. It will examine the writings of indigenous and marginalized groups which have not yet been studied.