Between 1896 and 1965 the Overseas Nursing Association (ONA) recruited more than 8,000 British nurses to work throughout the British Empire. This London-based imperial philanthropic association had as its aim to disseminating western medicine, as well as a British model of the nursing profession, around the globe. Its records document the processes of recruitment, the movement of its agents around the empire, and the ongoing connections between these agents and the metropolitan center. Hundreds of these nurses were assigned to colonial and mandatory territories around the Middle East and Mediterranean, which formed a substantial part of this global and imperial nursing network. By applying western medical practices and regulating the lives of local populations, these women played a crucial role in the imperial project. While the ONA has received limited scholarly attention, most of its operation remains largely unexplored, particularly the history of its agents in the above regions. Based on official records and personal letters, the proposed presentation will trace the mobility of British nurses around the regions, and focuses on their roles and experiences in two particular territories: Palestine and Cyprus. Located in the margins of empire and administered as a Mandate Territory and a Crown Colony, the two study-cases present a variety of colonial governance, strategic importance, characteristics of local populations and contemporary circumstances. Rather than projecting a single image into many forms of colonial nursing, this comparative and trans-local account of colonial nursing allows to highlight its disparities as well as shared patterns across space and time and demonstrates its multifaceted nature.